STANDARD ES CELL MEDIA (600 ml) (F. Mariani 03/12/2003):
ES CELL PROTOCOLS (for ES Cells that are feeder independent)
473.4ml KNOCK-DMEM
90ml 15% FBS (HyClone Tested Lot)
6ml Pen-Strep (100X)
6ml Na Glutamine (100X)
12 ml LIF (50X)
6ml Na Pyruvate, 0.11mg/ml (100X)
6 ml Non-essential Amino Acids (100X)
0.6ml 2-mercaptoethanol, 5.5uM (1000X)
Add DMEM to top of 500ml filter unit. Attach vaccuum. Add the rest of the components as it is filtered. Store at 4 degrees.
Keep components in convenient aliquots.
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ORDERING INFORMATION:
DMEM 1X (500ml) SLM-12B
15% FBS (500ml)
Pen-Strep CCF FM100-400
Na Glutamine CCF ED200
LIF Made in-house
Na Pyruvate, 0.11mg/ml EM100
Non-essential Amino Acid TMS001-C
2-mercaptoethanol, 5.5uM (50ml) Gibco-BRL
PBS-pH7.2 Gibco-BRL
ES CELL PROTOCOLS (for ES Cells that are feeder independent)
473.4ml KNOCK-DMEM
90ml 15% FBS (HyClone Tested Lot)
6ml Pen-Strep (100X)
6ml Na Glutamine (100X)
12 ml LIF (50X)
6ml Na Pyruvate, 0.11mg/ml (100X)
6 ml Non-essential Amino Acids (100X)
0.6ml 2-mercaptoethanol, 5.5uM (1000X)
Add DMEM to top of 500ml filter unit. Attach vaccuum. Add the rest of the components as it is filtered. Store at 4 degrees.
Keep components in convenient aliquots.
__________________________________________________________________
ORDERING INFORMATION:
DMEM 1X (500ml) SLM-12B
15% FBS (500ml)
Pen-Strep CCF FM100-400
Na Glutamine CCF ED200
LIF Made in-house
Na Pyruvate, 0.11mg/ml EM100
Non-essential Amino Acid TMS001-C
2-mercaptoethanol, 5.5uM (50ml) Gibco-BRL
PBS-pH7.2 Gibco-BRL